DNA

Part:BBa_K4361001:Design

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-08-21)


BlcR-binding oligo, 51 bp, wild type


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Ensure that this part is compatible with BioBrick standards


Source

-Naturally found in Agrobacterium tumefaciens strain C58

-First identified by Chai et al. in 2007, further characterized by Pan et al. in 2011

References

Chai, Y., Tsai, C. S., Cho, H., & Winans, S. C. (2007). Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens. Journal of bacteriology, 189(9), 3674–3679. https://doi.org/10.1128/JB.01274-06

Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C., & Chen, L. (2011). TheAgrobacterium tumefaciensTranscription Factor BlcR Is Regulated via Oligomerization. Journal of Biological Chemistry, 286(23), 20431–20440. https://doi.org/10.1074/jbc.m110.196154